The Determination of Serum Acid and Alkaline Phosphatase Activity with 4-Aminoantipyrine (A.A.P.)

The determination of serum acid and alkaline phosphatase activity with 4-aminoantipyrine (A.A.P.).

C6H5 Gottlieb and Marsh (1946), using alka:ine ferricyanide as the oxidant, devised a method for the estimation of certain phenolic fungicides with A.A.P. The procedure was modified by Grifols (1951) for the determination of phenol in the measurement of alkaline phosphatase activity in body fluids. This author made a careful study of the factors which control the development of the colour and h...

Rapid method for the quantitative determination of serum alkaline phosphatase.

S ERUM ALKALINE PHOSPHATASE ACTIVITY is the enzyme analysis most frequently made in clinical laboratories. It has long been a diagnostic tool and therapeutic index in both liver and osseous disease. Its usefulness could be expanded by a method which would measure alkaline phosphatase activity rapidly, accurately, and with a minimum of technical manipulation. Ideally, the substrate for such a me...

Skeletal alkaline phosphatase activity in serum.

Depression of serum alkaline phosphatase activity by human serum albumin.

The daily intravenous administration of 50 gm. of concentrated saltpoor human serum albumin’ produces a rise in serum albumin level and a decline in urinary calcium and phosphorus excretion (1). These latter changes have been interpreted as evidence for an albumin-induced stimulation of osteogenesis (2). The consistent finding of a concomitant depression of serum alkaline phosphatase activity w...

The Estimation of Serum Inorganic Phosphate and “acid” and “alkaline” Phosphatase Activity*

In 1930, Kay (6, 7) introduced a method for the quantitative determination of “alkaline” phosphatase activity of blood, employing sodium P-glycerophosphate as the substrate. Of the many modifications which have been introduced in which this substrate is employed, Bodansky’s (3, 4) method seems to have found the greatest usage because of its short incubation period and its accuracy. For our stud...